Description
Rat BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit
The Rat BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit is designed for the precise quantitative analysis of Bone Morphogenetic Protein Receptor II levels in a variety of rat biological samples. As an essential component of the bone morphogenetic protein (BMP) signaling pathway, BMPR2 plays a pivotal role in cellular responses to BMP ligands, influencing processes such as cell proliferation, differentiation, and development. The receptor's function is crucial in mediating the effects of BMPs on a wide range of physiological and pathological conditions, including skeletal growth, organogenesis, and disease mechanisms.
This advanced ELISA kit from Assay Genie offers outstanding sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under strict quality control measures, this kit provides robust performance, reliability, and ease of use, making it an excellent tool for researchers investigating the intricate interactions of BMPR2 in various biological contexts. Dive into the realm of BMP signaling pathways with confidence using this cutting-edge ELISA kit.
| Product Name: | Rat BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit |
| Product Code: | AEES00329 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 78.13-5000 pg/mL |
| Sensitivity: | 46.88 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat BMPR2 in samples. No significant cross-reactivity or interference between Rat BMPR2 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat BMPR2 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMPR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMPR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMPR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BMPR2. You can calculate the concentration of Rat BMPR2 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BMPR2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BMPR2 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 246.56 | 521.74 | 2143.23 | 223.28 | 523.68 | 1969.38 |
| Standard deviation | 15.19 | 22.12 | 69.23 | 12.93 | 29.43 | 70.70 |
| CV (%) | 6.16 | 4.24 | 3.23 | 5.79 | 5.62 | 3.59 |
Recovery
The recovery of Rat BMPR2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-102 | 95 |
| EDTA plasma (n=8) | 91-105 | 98 |
| Cell culture media (n=8) | 94-105 | 99 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
