The Rat BSP (Bone Sialoprotein) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of rat BSP levels in biological samples. BSP is a key protein in bone formation and remodeling processes, making it a valuable marker for studying bone-related diseases and conditions.This kit offers reliable and reproducible results, making it suitable for a variety of research applications in the fields of orthopedics, bone biology, and musculoskeletal research.
By measuring BSP levels, researchers can gain valuable insights into bone metabolism, fracture healing, and the development of osteoporosis.Overall, the Rat BSP CLIA Kit is a powerful tool for investigating the role of BSP in bone health and disease, providing researchers with a valuable resource for advancing their scientific studies in this important area of research.
Product Name:
Rat BSP (Bone Sialoprotein) CLIA Kit
SKU:
RTES00146
Target:
Rat BSP (Bone Sialoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BSP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BSP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BSP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BSP. You can calculate the concentration of Rat BSP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
92-109
89-105
Average (%)
93
99
96
1:4
Range (%)
99-117
97-112
97-111
Average (%)
107
105
103
1:8
Range (%)
90-103
89-100
102-114
Average (%)
98
95
108
1:16
Range (%)
94-105
94-104
101-114
Average (%)
100
99
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
100-114
106
Cell culture media (n=5)
87-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.04
3.63
0.51
1.02
3.28
Standard deviation
0.06
0.12
0.28
0.06
0.07
0.35
C V (%)
11.32
11.54
7.71
11.76
6.86
10.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BSP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BSP in samples. No significant cross-reactivity or interference between Rat BSP and analogues was observed.
