The Rat BTA (Bladder Tumor Antigen) CLIA Kit (RTES00064) from Assay Genie is a cutting-edge diagnostic tool designed for the accurate and efficient detection of bladder tumor antigen levels in rat samples. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.Bladder tumor antigen is a key marker in the study of bladder cancer and other urological disorders, playing a crucial role in early detection and monitoring of disease progression.
By accurately measuring BTA levels in rat samples, researchers can gain valuable insights into the development and progression of bladder tumors, leading to advancements in diagnosis and treatment strategies. Trust the Rat BTA CLIA Kit from Assay Genie to provide precise and reliable results for your research needs in the field of urology and oncology. Order now and take the next step towards groundbreaking discoveries in bladder cancer research.
Product Name:
Rat BTA (Bladder Tumor Antigen) CLIA Kit
SKU:
RTES00064
Target:
Rat BTA (Bladder Tumor Antigen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 ng/mL
Detection range:
125.00-8000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BTA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BTA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BTA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BTA. You can calculate the concentration of Rat BTA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-99
91-102
97-113
Average (%)
90
97
104
1:4
Range (%)
87-102
95-108
86-102
Average (%)
94
100
93
1:8
Range (%)
97-114
84-97
90-105
Average (%)
104
90
97
1:16
Range (%)
89-99
101-119
102-117
Average (%)
94
108
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
90-106
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
374.94
1075.52
3781.78
402.53
986.75
4047.06
Standard deviation
31.46
121.32
421.29
45.53
95.81
368.69
C V (%)
8.39
11.28
11.14
11.31
9.71
9.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BTA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BTA in samples. No significant cross-reactivity or interference between Rat BTA and analogues was observed.
