The Rat CA2 (Carbonic Anhydrase II) CLIA Kit is specifically designed for the quantitative detection of CA2 levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and precision, providing accurate and reliable results for various research applications.CA2 is an important enzyme that plays a key role in maintaining acid-base balance in the body, particularly in the kidneys and lungs. It is also involved in various physiological processes such as bone resorption, calcification, and pH regulation.
Abnormal CA2 levels have been linked to diseases like osteoporosis, renal tubular acidosis, and glaucoma, making it a valuable biomarker for studying these conditions and potential treatment strategies.Overall, the Rat CA2 CLIA Kit is a valuable tool for researchers studying the role of CA2 in health and disease, offering precise and consistent results for experimental investigations.
Product Name:
Rat CA2 (Carbonic Anhydrase II) CLIA Kit
SKU:
RTES00087
Target:
Rat CA2 (Carbonic Anhydrase II)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CA2. You can calculate the concentration of Rat CA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
89-105
100-112
Average (%)
105
96
106
1:4
Range (%)
87-101
101-117
97-110
Average (%)
94
107
104
1:8
Range (%)
98-114
87-100
85-96
Average (%)
104
93
91
1:16
Range (%)
90-100
97-111
98-116
Average (%)
95
104
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
99-113
105
Cell culture media (n=5)
100-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.41
3.75
13.27
1.28
3.73
14.41
Standard deviation
0.16
0.4
0.89
0.1
0.39
1.07
C V (%)
11.35
10.67
6.71
7.81
10.46
7.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CA2 in samples. No significant cross-reactivity or interference between Rat CA2 and analogues was observed.
