The Rat Calu (Calumenin) CLIA Kit is specifically designed for the quantitative detection of calumenin levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, allowing for precise and accurate results for a variety of research applications.Calumenin is a calcium-binding protein that plays a crucial role in cell signaling and regulation of calcium homeostasis. It is involved in various cellular processes such as cell adhesion, migration, and differentiation.
Dysregulation of calumenin has been linked to diseases such as cancer, diabetes, and Alzheimer's, making it a valuable biomarker for understanding these conditions and developing potential therapeutic interventions.Overall, the Rat Calu (Calumenin) CLIA Kit provides researchers with a reliable tool for studying the role of calumenin in physiological and pathological processes, offering valuable insights into disease mechanisms and potential treatment strategies.
Product Name:
Rat CALU (Calumenin) CLIA Kit
SKU:
RTES00084
Target:
Rat CALU (Calumenin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CALU. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CALU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CALU, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CALU. You can calculate the concentration of Rat CALU in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
98-112
99-114
Average (%)
95
103
107
1:4
Range (%)
96-110
92-104
98-113
Average (%)
102
97
104
1:8
Range (%)
99-114
99-117
88-98
Average (%)
106
107
93
1:16
Range (%)
91-102
85-100
97-111
Average (%)
97
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
100
EDTA plasma (n=5)
98-114
104
Cell culture media (n=5)
98-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.21
3.44
0.49
1.25
3.33
Standard deviation
0.05
0.09
0.33
0.04
0.12
0.31
C V (%)
9.43
7.44
9.59
8.16
9.6
9.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CALU concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CALU in samples. No significant cross-reactivity or interference between Rat CALU and analogues was observed.
