Description
Rat CAM (Calmodulin) ELISA Kit
The Rat CAM (Calmodulin) ELISA Kit is expertly designed to enable the quantitative detection of calmodulin levels in various biological samples. Calmodulin, a ubiquitous calcium-binding protein, plays a pivotal role in various cellular processes. It acts as a crucial regulator of calcium-dependent signal transduction pathways, impacting functions such as muscle contraction, cell proliferation, synaptic transmission, and gene expression. Accurate measurement of calmodulin levels is essential for unraveling its involvement in physiological and pathological conditions.
The ELISA kit offers exceptional sensitivity and specificity, allowing for precise and reproducible results. Manufactured with rigorous quality control standards, this kit ensures robust performance and user-friendly operation, making it an ideal choice for research applications where the role of calmodulin is of interest.
| Product Name: | Rat CAM (Calmodulin) ELISA Kit |
| Product Code: | AEES00338 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.10 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat CAM in samples. No significant cross-reactivity or interference between Rat CAM and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat CAM concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CAM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CAM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CAM. You can calculate the concentration of Rat CAM in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CAM were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CAM were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.50 | 1.10 | 3.50 | 0.50 | 1.20 | 3.50 |
| Standard deviation | 0.04 | 0.06 | 0.11 | 0.03 | 0.05 | 0.12 |
| CV (%) | 8.00 | 5.45 | 3.14 | 6.00 | 4.17 | 3.43 |
Recovery
The recovery of Rat CAM spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-104 | 95 |
| EDTA plasma (n=8) | 86-98 | 93 |
| Cell culture media (n=8) | 90-104 | 96 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
