Rat Carnitine Palmitoyltransferase 1A, Liver (CPT1A) ELISA Kit
The Rat Carnitine Palmitoyltransferase 1A (Liver CPT1A) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of CPT1A levels in rat liver samples. This kit is reliable and reproducible, providing researchers with precise results for a variety of experimental settings.Carnitine Palmitoyltransferase 1A is a key enzyme in fatty acid oxidation, playing a crucial role in energy metabolism and lipid homeostasis. Dysregulation of CPT1A has been implicated in various metabolic disorders, making it a valuable target for studying metabolic diseases and developing potential interventions.
The Rat Carnitine Palmitoyltransferase 1A (Liver CPT1A) ELISA Kit is essential for investigating the role of CPT1A in metabolic pathways, assessing its expression in different disease states, and exploring potential therapeutic strategies. It is a valuable tool for researchers working in the fields of metabolism, energy regulation, and metabolic disorders.
Product Name:
Rat Carnitine Palmitoyltransferase 1A, Liver (CPT1A) ELISA Kit
Product Code:
RTDL00245
Size:
96 Assays
Target:
CPT1A
Synonyms:
CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
