Rat Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) ELISA Kit
The Rat Carnitine Palmitoyltransferase-2 (Mitochondrial CPT2) ELISA Kit is a powerful tool for researchers looking to study this important enzyme in rat samples. This kit is specifically designed for the accurate quantification of CPT2 levels in rat serum, plasma, and tissue lysates with high sensitivity and specificity.CPT2 is a key enzyme involved in fatty acid beta-oxidation in mitochondria, playing a crucial role in energy metabolism and lipid homeostasis.
Dysregulation of CPT2 has been linked to various metabolic disorders, making it a valuable target for understanding these conditions and developing potential therapeutic interventions.With reliable and reproducible results, the Rat CPT2 ELISA Kit is suitable for a wide range of research applications, providing valuable insights into the role of CPT2 in health and disease in rat models.
Product Name:
Rat Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) ELISA Kit
Product Code:
RTDL00246
Size:
96 Assays
Target:
CPT2
Synonyms:
CPTASE, Carnitine O-Palmitoyltransferase 2, Carnitine palmitoyltransferase II
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
