The Rat Caspase-3 CLIA kit is a powerful tool for the precise measurement of caspase-3 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Caspase-3 is a key enzyme involved in apoptotic cell death, playing a critical role in regulating cell survival and programmed cell death processes. Dysregulation of caspase-3 activity has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. Therefore, accurate measurement of caspase-3 levels is essential for understanding disease mechanisms and developing potential therapeutic interventions.
The Rat Caspase-3 CLIA kit from Assay Genie provides researchers with a reliable and efficient method for studying caspase-3 biology in rat models. Its high sensitivity and specificity make it a valuable tool for investigating apoptosis pathways, identifying potential drug targets, and exploring novel treatment strategies for a wide range of diseases. Trust Assay Genie for accurate and dependable results in your caspase-3 research studies.
Product Name:
Rat CASP3 (Caspase 3) CLIA Kit
SKU:
RTES00096
Target:
Rat CASP3 (Caspase 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CASP3. You can calculate the concentration of Rat CASP3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-118
99-113
100-117
Average (%)
110
106
108
1:4
Range (%)
93-107
97-114
98-110
Average (%)
100
105
104
1:8
Range (%)
90-106
100-117
98-113
Average (%)
97
106
103
1:16
Range (%)
89-102
89-101
99-112
Average (%)
96
96
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
100-114
108
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.33
540.16
1769.87
198.15
506.44
1937.95
Standard deviation
21.34
57.91
160.35
17.89
51.4
122.09
C V (%)
11.64
10.72
9.06
9.03
10.15
6.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CASP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASP3 in samples. No significant cross-reactivity or interference between Rat CASP3 and analogues was observed.
