The Rat Caspase-7 CLIA Kit is a highly sensitive and specific assay designed for the precise detection of rat caspase-7 levels in various biological samples. This kit offers accurate and reproducible results, making it suitable for a wide range of research applications.Caspase-7 is a key enzyme in the apoptotic pathway, playing a critical role in programmed cell death. Dysregulation of caspase-7 has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
Therefore, measuring caspase-7 levels can provide valuable insights into disease progression and potential therapeutic targets.With its high performance and reliability, the Rat Caspase-7 CLIA Kit is an essential tool for scientists and researchers studying apoptotic pathways and exploring the role of caspase-7 in disease development and progression.
Product Name:
Rat CASP7 (Caspase 7) CLIA Kit
SKU:
RTES00097
Target:
Rat CASP7 (Caspase 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CASP7. You can calculate the concentration of Rat CASP7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
89-103
91-106
Average (%)
98
96
99
1:4
Range (%)
97-112
89-100
93-106
Average (%)
104
94
98
1:8
Range (%)
95-110
99-114
99-116
Average (%)
102
106
107
1:16
Range (%)
85-100
101-120
90-104
Average (%)
92
109
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
104
EDTA plasma (n=5)
96-111
102
Cell culture media (n=5)
83-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.68
577.24
1488.69
201.15
577.65
1572.37
Standard deviation
23.44
64.88
90.07
20.64
57.82
170.6
C V (%)
11.8
11.24
6.05
10.26
10.01
10.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CASP7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASP7 in samples. No significant cross-reactivity or interference between Rat CASP7 and analogues was observed.
