The Rat Casp8 (Caspase-8) CLIA Kit is specifically designed for the sensitive and accurate detection of Caspase-8 levels in rat samples such as serum, plasma, and tissue homogenates. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Caspase-8 plays a crucial role in cell apoptosis and inflammation, making it a key protein in various physiological and pathological processes.
Studies have shown its involvement in immune response regulation, cell death signaling, and cancer progression, highlighting its importance as a potential therapeutic target and biomarker for disease diagnosis and treatment.Overall, the Rat Casp8 CLIA Kit offers researchers a valuable tool for studying Caspase-8 biology and its implications in health and disease, providing accurate and precise measurements for insightful research outcomes.
Product Name:
Rat CASP8 (Caspase 8) CLIA Kit
SKU:
RTES00156
Target:
Rat CASP8 (Caspase 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CASP8. You can calculate the concentration of Rat CASP8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
98-109
85-99
Average (%)
104
103
92
1:4
Range (%)
88-103
102-120
88-104
Average (%)
94
110
95
1:8
Range (%)
85-99
86-102
102-116
Average (%)
90
93
109
1:16
Range (%)
96-111
95-111
102-119
Average (%)
102
101
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
99
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.52
244.8
923.64
101.3
267.27
921.44
Standard deviation
10.58
25.95
69.27
10.05
27.34
67.45
C V (%)
9.66
10.6
7.5
9.92
10.23
7.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CASP8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CASP8 in samples. No significant cross-reactivity or interference between Rat CASP8 and analogues was observed.
