The Rat CAV-1 (Caveolin-1) CLIA Kit is a powerful tool for detecting caveolin-1 levels in rat samples with high precision and accuracy. This innovative kit is specifically designed for use with rat serum, plasma, and tissue homogenates, providing researchers with reliable and reproducible results for a variety of experimental applications.Caveolin-1 is a key protein involved in cell signaling and membrane organization, playing a critical role in various cellular processes such as vesicular trafficking, cholesterol homeostasis, and signal transduction. Dysregulation of caveolin-1 expression has been linked to several diseases, including cancer, cardiovascular disorders, and metabolic syndrome, highlighting the importance of studying this protein as a potential biomarker and therapeutic target.
With its exceptional sensitivity and specificity, the Rat CAV-1 CLIA Kit offers researchers a valuable tool for investigating caveolin-1 biology and its implications in disease pathogenesis. Trust in this reliable kit for accurate quantification and analysis of caveolin-1 levels in rat samples, advancing your research and discovery efforts.
Product Name:
Rat CAV-1 (Caveolin-1) CLIA Kit
SKU:
RTES00104
Target:
Rat CAV-1 (Caveolin-1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat Cav-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Cav-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Cav-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat Cav-1. You can calculate the concentration of Rat Cav-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
99-115
98-116
Average (%)
103
107
106
1:4
Range (%)
85-97
99-116
95-109
Average (%)
90
107
102
1:8
Range (%)
90-104
91-106
93-106
Average (%)
98
98
98
1:16
Range (%)
98-113
100-116
100-118
Average (%)
105
108
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-113
104
EDTA plasma (n=5)
97-111
105
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.44
185.76
977.04
88.39
184.61
1000.82
Standard deviation
11.98
13.02
58.82
10.22
21.4
75.16
C V (%)
12.29
7.01
6.02
11.56
11.59
7.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat Cav-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Cav-1 in samples. No significant cross-reactivity or interference between Rat Cav-1 and analogues was observed.
