The Rat CCKAR (Cholecystokinin A Receptor) CLIA Kit is a highly sensitive and specific assay designed for the quantitative measurement of CCKAR levels in rat samples, including serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an ideal tool for a variety of research applications.The cholecystokinin A receptor (CCKAR) is a key component in the regulation of digestive processes and plays a crucial role in the gastrointestinal system.
Dysregulation of CCKAR has been implicated in various digestive disorders and metabolic conditions, highlighting its importance as a potential therapeutic target.With its high sensitivity and specificity, the Rat CCKAR CLIA Kit offers reliable data for studying the role of CCKAR in health and disease, aiding in the development of new treatments and interventions in the field of digestive health and metabolism.
Product Name:
Rat CCKAR (Cholecystokinin A Receptor) CLIA Kit
SKU:
RTES00114
Target:
Rat CCKAR (Cholecystokinin A Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CCKAR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CCKAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CCKAR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CCKAR. You can calculate the concentration of Rat CCKAR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
97-110
98-113
Average (%)
108
104
105
1:4
Range (%)
102-115
92-106
87-100
Average (%)
108
100
94
1:8
Range (%)
92-109
92-109
91-106
Average (%)
100
100
98
1:16
Range (%)
96-109
103-119
88-103
Average (%)
102
109
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
104
EDTA plasma (n=5)
97-110
103
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.19
226.96
829.93
90.35
218.98
791.15
Standard deviation
9.7
16.66
79.18
11.18
17.01
56.57
C V (%)
10.52
7.34
9.54
12.37
7.77
7.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CCKAR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CCKAR in samples. No significant cross-reactivity or interference between Rat CCKAR and analogues was observed.
