Rat CCRL1 (Chemokine C-C-Motif Receptor Like Protein 1) CLIA Kit
The Rat CCRL1 (Chemokine C-C motif receptor-like protein 1) CLIA Kit is a powerful tool for the quantitative detection of CCRL1 levels in rat samples. This kit offers exceptional sensitivity and specificity, providing accurate and consistent results for a variety of research applications.CCRL1 is a cell surface protein that plays a role in immune response and inflammation by binding to chemokines. It is involved in regulating leukocyte migration and activation, making it a key player in the immune system.
Understanding CCRL1 levels can provide valuable insights into immune-related diseases and therapeutic interventions.The Rat CCRL1 CLIA Kit from AssayGenie is an essential tool for researchers studying immune response, inflammation, and chemokine signaling in rat models. With its high performance and reliability, this kit is an indispensable resource for advancing scientific knowledge and developing novel treatments for immune-related conditions.
Product Name:
Rat CCRL1 (Chemokine C-C-Motif Receptor Like Protein 1) CLIA Kit
SKU:
RTES00107
Target:
Rat CCRL1 (Chemokine C-C-Motif Receptor Like Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CCRL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CCRL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CCRL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CCRL1. You can calculate the concentration of Rat CCRL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
99-112
88-104
Average (%)
101
106
95
1:4
Range (%)
90-105
91-107
87-100
Average (%)
98
99
93
1:8
Range (%)
92-104
87-100
88-102
Average (%)
97
92
95
1:16
Range (%)
95-107
101-114
86-98
Average (%)
101
107
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
98-109
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.28
108.38
495.86
58.27
113.78
505.66
Standard deviation
4.52
11.69
46.02
4.95
11.03
35.5
C V (%)
8.18
10.79
9.28
8.49
9.69
7.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CCRL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CCRL1 in samples. No significant cross-reactivity or interference between Rat CCRL1 and analogues was observed.
