The Rat CD3 (Cluster of Differentiation 3) CLIA Kit is a powerful tool for quantitative analysis of CD3 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.CD3 is a cell surface marker essential for T cell function and plays a critical role in the immune response. Abnormal levels of CD3 have been linked to autoimmune disorders, infections, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing targeted therapies.
With its advanced technology and ease of use, the Rat CD3 CLIA Kit from AssayGenie is the perfect choice for researchers looking to explore the role of CD3 in immune system regulation and disease pathology. Unlock new insights and drive innovation with this cutting-edge assay kit.
Product Name:
Rat CD3 (Cluster of Differentiation 3) CLIA Kit
SKU:
RTES00122
Target:
Rat CD3 (Cluster of Differentiation 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CD3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CD3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CD3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CD3. You can calculate the concentration of Rat CD3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
98-113
97-114
Average (%)
95
104
104
1:4
Range (%)
102-118
94-106
93-106
Average (%)
108
99
100
1:8
Range (%)
87-103
100-112
91-106
Average (%)
94
107
98
1:16
Range (%)
97-111
90-104
90-104
Average (%)
104
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
106
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.36
462.31
1469.2
192.19
455.17
1511.32
Standard deviation
16.8
45.58
118.86
17.41
54.12
154.15
C V (%)
8.22
9.86
8.09
9.06
11.89
10.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CD3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CD3 in samples. No significant cross-reactivity or interference between Rat CD3 and analogues was observed.
