Rat CD40L (Cluster Of Differentiation 40 Ligand) CLIA Kit
The Rat CD40L (Cluster of Differentiation 40 Ligand) CLIA Kit is a powerful tool for accurately measuring CD40L levels in rat samples. This kit is designed for use with serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for reliable and reproducible results.CD40L is a key protein involved in immune responses and inflammatory processes, making it a valuable biomarker for studying autoimmune diseases, inflammatory conditions, and vaccine development.
By accurately quantifying CD40L levels, researchers can gain insight into the immune system's activity and potential therapeutic targets.With its easy-to-use format and high-quality components, the Rat CD40L CLIA Kit is an essential tool for researchers studying immune regulation and inflammation in rat models. Get accurate and reliable results for your research applications with this innovative kit from Assay Genie.
Product Name:
Rat CD40L (Cluster Of Differentiation 40 Ligand) CLIA Kit
SKU:
RTES00124
Target:
Rat CD40L (Cluster Of Differentiation 40 Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CD40L. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CD40L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CD40L, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CD40L. You can calculate the concentration of Rat CD40L in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
95-109
91-106
Average (%)
104
103
96
1:4
Range (%)
100-117
99-113
86-101
Average (%)
107
105
93
1:8
Range (%)
90-101
90-103
91-103
Average (%)
96
97
97
1:16
Range (%)
97-114
95-109
99-110
Average (%)
105
100
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
96-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.64
225.61
873.02
95.15
214.21
837.06
Standard deviation
12.45
16.33
99.52
8.54
22.06
71.32
C V (%)
12.49
7.24
11.4
8.98
10.3
8.52
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CD40L concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CD40L in samples. No significant cross-reactivity or interference between Rat CD40L and analogues was observed.
