Description
Rat CD40L (Cluster Of Differentiation 40 Ligand) ELISA Kit
The Rat CD40L (Cluster of Differentiation 40 Ligand) ELISA Kit is a cutting-edge assay meticulously designed for the precise quantitative determination of CD40L levels in various rat biological samples. CD40L, also known as CD154, is a key cell surface molecule involved in immune regulation, particularly in T cell-dependent B cell activation and function. This ELISA kit serves as a pivotal tool for researchers aiming to investigate the role of CD40L in immune responses, inflammation, and the development of immunological disorders. By accurately measuring CD40L levels, researchers can unravel its critical functions in modulating adaptive immune responses, inflammation, and antibody production.
CD40L dysregulation has been linked to autoimmune diseases, inflammatory conditions, and impaired immune responses, underscoring the significance of precise measurements to advance the understanding of immune-related mechanisms. The Rat CD40L ELISA Kit by Assay Genie is crafted to deliver exceptional sensitivity and specificity, ensuring trustworthy and reproducible results. This kit, manufactured under stringent quality controls, offers robust performance and user-friendly protocols, making it an optimal choice for comprehensive research endeavors exploring the intricate role of CD40L in immune modulation and immunopathology.
| Product Name: | Rat CD40L (Cluster Of Differentiation 40 Ligand) ELISA Kit |
| Product Code: | AEES00352 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.10 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat CD40L in samples. No significant cross-reactivity or interference between Rat CD40L and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat CD40L concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CD40L. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CD40L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CD40L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CD40L. You can calculate the concentration of Rat CD40L in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CD40L were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CD40L were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.54 | 0.88 | 4.42 | 0.57 | 0.81 | 4.28 |
| Standard deviation | 0.03 | 0.05 | 0.14 | 0.03 | 0.04 | 0.13 |
| CV (%) | 5.56 | 5.68 | 3.17 | 5.26 | 4.94 | 3.04 |
Recovery
The recovery of Rat CD40L spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-110 | 100 |
| EDTA plasma (n=8) | 89-101 | 96 |
| Cell culture media (n=8) | 93-107 | 100 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
