The Rat CD8 (Cluster of Differentiation 8) CLIA Kit is a precise tool designed for the detection of CD8 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an essential resource for various research applications.CD8 is a critical marker for cytotoxic T cells, playing a key role in the immune response against infections and diseases.
Monitoring CD8 levels can provide valuable insights into immune function and response, offering potential applications in immunology, oncology, and infectious disease research.This kit from AssayGenie is a reliable option for researchers looking to study CD8 expression and function in rat models, offering a valuable tool for advancing our understanding of immune responses and developing novel therapeutic strategies.
Product Name:
Rat CD8 (Cluster of Differentiation 8) CLIA Kit
SKU:
RTES00125
Target:
Rat CD8 (Cluster of Differentiation 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CD8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CD8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CD8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CD8. You can calculate the concentration of Rat CD8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
86-98
103-115
Average (%)
90
92
108
1:4
Range (%)
97-109
91-104
88-101
Average (%)
103
98
94
1:8
Range (%)
97-113
95-107
91-105
Average (%)
105
100
99
1:16
Range (%)
100-112
94-108
89-103
Average (%)
106
101
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
97-111
104
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
1.84
8.04
0.89
2.02
8.21
Standard deviation
0.11
0.16
0.79
0.08
0.19
0.61
C V (%)
11.7
8.7
9.83
8.99
9.41
7.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CD8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CD8 in samples. No significant cross-reactivity or interference between Rat CD8 and analogues was observed.
