The Rat CD97 (Cluster of Differentiation 97) CLIA Kit is specifically designed for the accurate and precise measurement of CD97 levels in rat samples. This kit offers high sensitivity and specificity, guaranteeing dependable and consistent results for various research purposes.CD97 is a cell surface glycoprotein that plays a vital role in cell adhesion and migration, making it a key player in various physiological and pathological processes. It is implicated in immune responses, inflammation, and cancer metastasis, making it a valuable biomarker for studying these conditions and exploring therapeutic strategies.
The Rat CD97 CLIA Kit from Assay Genie provides researchers with a reliable tool for investigating the role of CD97 in rat models, offering valuable insights into its functions and potential therapeutic applications. With its easy-to-use format and robust performance, this kit is an essential resource for studies focused on understanding the role of CD97 in health and disease.
Product Name:
Rat CD97 (Cluster of Differentiation 97) CLIA Kit
SKU:
RTES00126
Target:
Rat CD97 (Cluster of Differentiation 97)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CD97. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CD97 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CD97, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CD97. You can calculate the concentration of Rat CD97 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
85-96
100-114
Average (%)
108
91
107
1:4
Range (%)
96-109
96-111
100-114
Average (%)
101
102
106
1:8
Range (%)
95-113
87-102
103-117
Average (%)
103
94
109
1:16
Range (%)
99-111
87-103
100-117
Average (%)
104
94
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
98
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
96-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.41
59.16
200.43
27.78
61.94
192.96
Standard deviation
2.75
5.68
22.41
3.56
5.51
14.72
C V (%)
10.82
9.6
11.18
12.81
8.9
7.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CD97 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CD97 in samples. No significant cross-reactivity or interference between Rat CD97 and analogues was observed.
