Description
Rat CFB (Complement Factor B) ELISA Kit
The Rat CFB (Complement Factor B) ELISA Kit is a sophisticated and innovative tool meticulously designed for the quantitative determination of Complement Factor B levels in a variety of biological samples from rat subjects. Complement Factor B plays a key role in the alternative complement pathway, a crucial component of the immune system responsible for detecting and eliminating pathogens. Accurate measurement of CFB levels is vital for studying immune responses, complement activation, and their implications in various diseases. This ELISA kit provides researchers with a precise and reliable method to quantify Complement Factor B, enabling detailed insights into its functions, regulatory mechanisms, and involvement in immune-related processes.
Analyzing CFB levels can offer valuable information on immune surveillance, host defense, and immunopathological conditions. Understanding the quantitative dynamics of CFB is fundamental for elucidating the complex interplay between the complement system and immune responses. The Rat CFB ELISA Kit by Assay Genie boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under strict quality control measures, this kit delivers robust performance and user-friendly procedures, making it an optimal choice for cutting-edge research endeavors aiming to unravel the intricate mechanisms of the complement system and its pivotal role in immunity.
| Product Name: | Rat CFB (Complement Factor B) ELISA Kit |
| Product Code: | AEES00365 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 3.13-200 ng/mL |
| Sensitivity: | 1.88 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat CFB in samples. No significant cross-reactivity or interference between Rat CFB and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat CFB concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CFB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CFB. You can calculate the concentration of Rat CFB in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CFB were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CFB were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 10.06 | 25.92 | 81.01 | 9.11 | 25.03 | 80.09 |
| Standard deviation | 0.58 | 1.40 | 4.09 | 0.53 | 1.31 | 3.17 |
| CV (%) | 5.77 | 5.40 | 5.05 | 5.82 | 5.23 | 3.96 |
Recovery
The recovery of Rat CFB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-110 | 102 |
| EDTA plasma (n=8) | 92-106 | 98 |
| Cell culture media (n=8) | 89-103 | 94 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
