The Rat CFL1 (Cofilin-1) Non-Muscle CLIA Kit is a high-quality assay designed for the precise measurement of cofilin-1 levels in rat samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for various research applications.Cofilin-1 is a key protein involved in actin dynamics, playing a crucial role in cell migration, invasion, and cytoskeletal organization. Dysregulation of cofilin-1 has been implicated in various diseases, including cancer, neurological disorders, and cardiovascular conditions, making it a valuable biomarker for studying these pathologies and potential therapeutic interventions.
Overall, the Rat CFL1 (Cofilin-1) Non-Muscle CLIA Kit is an essential tool for researchers looking to investigate the role of cofilin-1 in disease pathology and developing novel treatment strategies.
Product Name:
Rat CFL1 (Cofilin 1, Non-Muscle) CLIA Kit
SKU:
RTES00135
Target:
Rat CFL1 (Cofilin 1, Non-Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CFL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CFL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CFL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CFL1. You can calculate the concentration of Rat CFL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
98-110
84-96
Average (%)
99
103
91
1:4
Range (%)
93-110
99-114
85-97
Average (%)
100
106
90
1:8
Range (%)
95-105
95-107
92-109
Average (%)
100
102
99
1:16
Range (%)
103-119
92-103
94-106
Average (%)
110
98
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
99
EDTA plasma (n=5)
94-110
100
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.38
244.85
988.68
101.98
253.71
1026.98
Standard deviation
8.2
17.97
72.67
12.43
22.76
67.06
C V (%)
8.42
7.34
7.35
12.19
8.97
6.53
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CFL1 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Rat CFL1 in samples. No significant cross-reactivity or interference between Rat CFL1 and analogues was observed.
