Rat CGRP (Calcitonin Gene Related Peptide) CLIA Kit
The Rat CGRP (Calcitonin Gene-Related Peptide) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CGRP levels in rat serum, plasma, and tissue extracts. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.CGRP is a neuropeptide that plays a key role in regulating pain transmission and inflammation. It is involved in a variety of physiological processes, including vasodilation, regulation of blood flow, and modulation of neuronal activity.
Dysregulation of CGRP levels has been implicated in various conditions such as migraine headaches, neurogenic inflammation, and neuropathic pain.By measuring CGRP levels in rat samples, researchers can gain valuable insights into the role of this neuropeptide in different disease states and potential therapeutic interventions. The Rat CGRP CLIA kit offers a powerful tool for studying the intricate mechanisms involving CGRP and advancing our understanding of its role in health and disease.
Product Name:
Rat CGRP (Calcitonin Gene Related Peptide) CLIA Kit
SKU:
RTES00077
Target:
Rat CGRP (Calcitonin Gene Related Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CGRP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CGRP1. You can calculate the concentration of Rat CGRP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
85-97
96-112
Average (%)
98
92
103
1:4
Range (%)
90-105
95-107
90-106
Average (%)
96
102
97
1:8
Range (%)
97-108
97-113
90-104
Average (%)
103
103
97
1:16
Range (%)
86-99
95-109
96-110
Average (%)
93
101
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.74
61.65
171.75
23.04
63.26
167.5
Standard deviation
2.63
6.25
14.32
2.01
4.57
11.71
C V (%)
10.63
10.14
8.34
8.72
7.22
6.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CGRP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CGRP1 in samples. No significant cross-reactivity or interference between Rat CGRP1 and analogues was observed.
