Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit
The Rat Chemokine C-X3-C Motif Ligand 1 (CX3CL1) ELISA Kit is a powerful tool for the quantitative measurement of CX3CL1 levels in rat samples. This kit is specifically designed to accurately detect CX3CL1 in serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for reliable results.CX3CL1, also known as fractalkine, is a unique chemokine that plays a crucial role in inflammation and immune responses. It is involved in the recruitment and activation of immune cells, and has been implicated in various diseases such as neuroinflammation, atherosclerosis, and cancer.
As a key mediator of immune cell trafficking and tissue inflammation, CX3CL1 is an important biomarker for studying these pathological processes.With its precise measurement capability and robust performance, the Rat CX3CL1 ELISA Kit is an essential tool for researchers looking to investigate the role of CX3CL1 in health and disease, as well as for potential therapeutic development.
Product Name:
Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
