Rat CHRM2 (Cholinergic Receptor, Muscarinic 2) CLIA Kit
The Rat CHRM2 (Cholinergic Receptor Muscarinic 2) CLIA Kit is designed for the precise measurement of CHRM2 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and consistent results for various research purposes.CHRM2 is a key receptor involved in cholinergic signaling, playing a critical role in various physiological processes, including neurotransmission and smooth muscle contraction.
Dysregulation of CHRM2 has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its reliable performance and versatility, the Rat CHRM2 CLIA Kit is an essential tool for researchers studying the role of cholinergic receptors in health and disease, providing valuable insights into potential therapeutic targets and treatment strategies.
Product Name:
Rat CHRM2 (Cholinergic Receptor, Muscarinic 2) CLIA Kit
SKU:
RTES00117
Target:
Rat CHRM2 (Cholinergic Receptor, Muscarinic 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pmol/mL
Detection range:
12.50-800 pmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CHRM2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CHRM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CHRM2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CHRM2. You can calculate the concentration of Rat CHRM2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
94-106
87-100
Average (%)
97
99
95
1:4
Range (%)
86-101
98-114
102-117
Average (%)
93
105
108
1:8
Range (%)
99-113
94-106
95-110
Average (%)
105
100
101
1:16
Range (%)
89-103
90-101
95-110
Average (%)
96
95
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
104
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
94-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.32
106.4
276.89
43.92
109.77
267.14
Standard deviation
3.56
8.83
30.6
3.92
10.81
26.05
C V (%)
8.41
8.3
11.05
8.93
9.85
9.75
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CHRM2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CHRM2 in samples. No significant cross-reactivity or interference between Rat CHRM2 and analogues was observed.
