Rat CLCF1 (Cardiotrophin Like Cytokine Factor 1) CLIA Kit
The Rat CLCF1 (Cardiotrophin-Like Cytokine Factor 1) CLIA Kit is a highly reliable and accurate tool for the measurement of CLCF1 levels in rat samples. This kit is designed for use with rat serum, plasma, and tissue homogenates, providing researchers with high sensitivity and specificity for dependable results.CLCF1 is a cytokine that plays a crucial role in various biological processes, including inflammation, cell differentiation, and immune response modulation. Dysregulation of CLCF1 has been linked to various diseases, making it a valuable biomarker for research into conditions such as autoimmune disorders, inflammatory diseases, and cancer.
With its robust performance and ease of use, the Rat CLCF1 CLIA Kit is an indispensable resource for conducting in-depth studies on the role of CLCF1 in health and disease, enabling researchers to gain valuable insights into potential therapeutic targets and personalized medicine approaches.
Product Name:
Rat CLCF1 (Cardiotrophin Like Cytokine Factor 1) CLIA Kit
SKU:
RTES00093
Target:
Rat CLCF1 (Cardiotrophin Like Cytokine Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CLCF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CLCF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CLCF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CLCF1. You can calculate the concentration of Rat CLCF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
89-103
103-118
Average (%)
105
94
109
1:4
Range (%)
98-113
101-117
99-112
Average (%)
104
109
106
1:8
Range (%)
89-103
92-105
100-113
Average (%)
95
97
106
1:16
Range (%)
102-121
101-116
87-100
Average (%)
110
108
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
91-104
98
Cell culture media (n=5)
97-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.66
103.29
333.61
35.03
108.56
335.24
Standard deviation
3.5
11.02
25.82
2.92
8.58
30.14
C V (%)
9.05
10.67
7.74
8.34
7.9
8.99
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CLCF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CLCF1 in samples. No significant cross-reactivity or interference between Rat CLCF1 and analogues was observed.
