The Rat CLU (Clusterin) CLIA Kit is specifically designed for the precise measurement of clusterin levels in rat serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Clusterin, also known as apolipoprotein J, is a multifunctional glycoprotein involved in various physiological processes, including cell apoptosis, lipid transportation, and tissue remodeling. It is associated with a wide range of diseases, including neurodegenerative disorders, cancer, and kidney disease, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.
Overall, the Rat CLU (Clusterin) CLIA Kit is an essential tool for researchers studying clusterin biology and its implications in health and disease. Its reliable performance and ease of use make it suitable for a wide range of laboratory settings and applications.
Product Name:
Rat CLU (Clusterin) CLIA Kit
SKU:
RTES00127
Target:
Rat CLU (Clusterin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CLU. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CLU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CLU, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CLU. You can calculate the concentration of Rat CLU in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
89-103
100-113
Average (%)
102
96
105
1:4
Range (%)
99-113
102-116
102-115
Average (%)
106
108
109
1:8
Range (%)
99-114
97-112
95-109
Average (%)
106
104
101
1:16
Range (%)
89-103
83-99
84-95
Average (%)
94
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
98-113
104
Cell culture media (n=5)
94-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.0
2.52
7.73
1.04
2.31
8.42
Standard deviation
0.12
0.28
0.83
0.12
0.25
0.52
C V (%)
12.0
11.11
10.74
11.54
10.82
6.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CLU concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CLU in samples. No significant cross-reactivity or interference between Rat CLU and analogues was observed.
