Description
Rat CLU (Clusterin) ELISA Kit
The Rat CLU (Clusterin) ELISA Kit is a cutting-edge assay engineered to quantitatively measure the levels of Clusterin in various rat biological samples. Clusterin, a versatile glycoprotein, plays diverse roles in cellular processes, ranging from apoptosis and lipid transport to stress response modulation.
This kit is meticulously designed to provide precise measurements of Clusterin expression, enabling researchers to gain insights into its functions in health, disease, and cellular homeostasis. Accurate quantification of Clusterin is vital for understanding its involvement in various physiological and pathological mechanisms. As a multifunctional protein, Clusterin contributes to cellular processes such as cell survival, apoptosis, and lipid metabolism. By accurately measuring Clusterin levels, researchers can uncover its roles in conditions including neurodegenerative diseases, cancer, and cardiovascular disorders, leading to potential therapeutic innovations and disease interventions. The Rat CLU ELISA Kit from Assay Genie is engineered to deliver exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under rigorous quality control standards, this kit offers robust performance and user-friendly operation, making it an ideal choice for research endeavors focusing on elucidating the functions and implications of Clusterin in biological systems.
| Product Name: | Rat CLU (Clusterin) ELISA Kit |
| Product Code: | AEES00355 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 1.56-100 ng/mL |
| Sensitivity: | 0.94 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100?L |
| Specificity: | This kit recognizes Rat CLU in samples. No significant cross-reactivity or interference between Rat CLU and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat CLU concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CLU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CLU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CLU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CLU. You can calculate the concentration of Rat CLU in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CLU were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CLU were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 4.79 | 13.90 | 49.99 | 5.20 | 15.07 | 50.52 |
| Standard deviation | 0.27 | 0.60 | 2.17 | 0.30 | 0.72 | 2.67 |
| CV (%) | 5.64 | 4.32 | 4.34 | 5.77 | 4.78 | 5.29 |
Recovery
The recovery of Rat CLU spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-98 | 92 |
| EDTA plasma (n=8) | 93-105 | 99 |
| Cell culture media (n=8) | 91-108 | 98 |
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| 1. | Add 100?L standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100?L Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100?L HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90?L Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50?L Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
