The Rat Col2 (Collagen Type 2) CLIA Kit is a highly sensitive assay designed for the accurate quantification of rat type 2 collagen levels in biological samples such as serum, plasma, and cell culture supernatants. This kit offers unparalleled specificity and precision, ensuring reliable and reproducible results for various research applications.Type 2 collagen is a key component of cartilage, playing a crucial role in maintaining the structural integrity and function of joints. Abnormalities in type 2 collagen metabolism have been linked to conditions such as osteoarthritis, rheumatoid arthritis, and other joint-related disorders.
Monitoring type 2 collagen levels can provide valuable insights into the progression of these diseases and aid in the development of targeted therapeutic interventions.The Rat Col2 (Collagen Type 2) CLIA Kit is an essential tool for researchers studying cartilage biology, skeletal development, and joint health. Its high sensitivity and accuracy make it a valuable resource for advancing our understanding of collagen biology and potentially identifying novel treatment strategies for joint-related disorders.
Product Name:
Rat COL2 (Collagen Type 2) CLIA Kit
SKU:
RTES00137
Target:
Rat COL2 (Collagen Type 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat COL2. You can calculate the concentration of Rat COL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
86-99
95-109
Average (%)
95
92
102
1:4
Range (%)
94-111
101-117
93-104
Average (%)
102
109
98
1:8
Range (%)
93-108
102-116
94-107
Average (%)
100
108
100
1:16
Range (%)
93-110
84-98
100-114
Average (%)
100
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-111
105
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
95-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.61
111.1
390.12
49.89
112.2
365.62
Standard deviation
5.22
10.44
30.12
4.68
9.73
34.51
C V (%)
10.31
9.4
7.72
9.38
8.67
9.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat COL2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL2 in samples. No significant cross-reactivity or interference between Rat COL2 and analogues was observed.
