Rat Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit
The Rat Colony Stimulating Factor 2 (CSF-2/GM-CSF) ELISA Kit is a highly accurate and reliable tool for measuring levels of CSF-2/GM-CSF in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit is perfect for a variety of research applications.CSF-2/GM-CSF is a key cytokine that plays a crucial role in regulating the immune response, particularly in the development and function of granulocytes, macrophages, and dendritic cells.
Dysregulation of CSF-2/GM-CSF has been linked to various inflammatory and autoimmune diseases, making it an important biomarker for studying these conditions and exploring potential treatments.Overall, the Rat Colony Stimulating Factor 2 (CSF-2/GM-CSF) ELISA Kit offers precise and reproducible results, making it an essential tool for researchers in the field of immunology and inflammation.
Product Name:
Rat Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
