Rat Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit
The Rat Colony Stimulating Factor 3 (Granulocyte GCSF) ELISA Kit is a powerful tool for the measurement of GCSF levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for accurate and reliable results to be obtained consistently.Granulocyte colony-stimulating factor (GCSF) is a key regulator of granulocyte production and function, playing a crucial role in immune response and inflammation. Monitoring GCSF levels can provide valuable insights into various physiological and pathological processes, making this ELISA kit indispensable for researchers investigating immunology, hematopoiesis, and inflammatory conditions in rat models.
With its user-friendly protocol and superior performance, the Rat Colony Stimulating Factor 3 (Granulocyte GCSF) ELISA Kit is an essential tool for advancing our understanding of granulocyte biology and exploring new therapeutic approaches for immune-related disorders in rats.
Product Name:
Rat Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
