The Rat CR (Calretinin) CLIA Kit is specifically designed for the precise measurement of calretinin levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Calretinin is a calcium-binding protein that plays a pivotal role in neuronal function and regulation. It is widely studied in the context of neurodegenerative diseases, such as Alzheimer's and Parkinson's, as well as in developmental neurobiology.
The Rat CR CLIA Kit provides a valuable tool for investigating the role of calretinin in these conditions and potential therapeutic interventions.Overall, the Rat CR CLIA Kit from Assay Genie is a reliable and versatile assay for researchers studying calretinin biology and its implications in various disease states.
Product Name:
Rat CR (Calretinin) CLIA Kit
SKU:
RTES00083
Target:
Rat CR (Calretinin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CR. You can calculate the concentration of Rat CR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
90-102
93-107
Average (%)
100
96
99
1:4
Range (%)
103-117
84-97
96-110
Average (%)
109
90
103
1:8
Range (%)
87-98
86-97
98-111
Average (%)
92
92
106
1:16
Range (%)
101-118
90-104
98-114
Average (%)
108
96
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
85-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.48
4.13
0.52
1.43
3.96
Standard deviation
0.04
0.15
0.37
0.05
0.15
0.33
C V (%)
8.16
10.14
8.96
9.62
10.49
8.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CR in samples. No significant cross-reactivity or interference between Rat CR and analogues was observed.
