Rat Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ELISA Kit
The Rat Cross-linked C-Telopeptide of Type I Collagen (CTXI) ELISA Kit is specifically designed for the precise measurement of CTXI levels in rat serum, plasma, and urine samples. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.CTXI is a key biomarker for bone metabolism and turnover, providing valuable insights into conditions such as osteoporosis, bone fractures, and skeletal disorders.
By measuring CTXI levels, researchers can better understand the dynamics of bone resorption and formation, contributing to the development of innovative strategies for diagnosing and treating bone-related diseases.Overall, the Rat CTXI ELISA Kit is a valuable tool for studying bone health and metabolism in rat models, offering reliable data to advance research in the fields of osteology and musculoskeletal biology.
Product Name:
Rat Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ELISA Kit
Product Code:
RTDL00269
Size:
96 Assays
Target:
CTXI
Synonyms:
CTX-I, CTX1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
