The Rat CRT (Calreticulin) CLIA Kit is a powerful tool for the precise measurement of calreticulin levels in rat samples, including serum and plasma. With its high sensitivity and specificity, this kit delivers reliable and consistent results, making it ideal for various research applications.Calreticulin is a multifunctional protein that plays a crucial role in various cellular processes, including protein folding, calcium homeostasis, and immune response regulation. Dysregulation of calreticulin has been linked to several diseases, including cancer, autoimmune disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and identifying potential therapeutic targets.
Overall, the Rat CRT (Calreticulin) CLIA Kit provides researchers with a valuable tool for studying calreticulin biology and its implications in disease pathology, offering a deeper understanding of potential diagnostic and therapeutic strategies.
Product Name:
Rat CRT (Calreticulin) CLIA Kit
SKU:
RTES00082
Target:
Rat CRT (Calreticulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CRT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CRT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CRT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CRT. You can calculate the concentration of Rat CRT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
93-105
90-102
Average (%)
97
99
97
1:4
Range (%)
96-110
98-115
103-118
Average (%)
103
106
110
1:8
Range (%)
85-95
89-100
101-117
Average (%)
90
94
108
1:16
Range (%)
98-109
90-103
96-111
Average (%)
103
97
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.66
111.14
338.03
49.52
106.52
345.16
Standard deviation
4.48
10.17
32.89
5.46
11.65
35.76
C V (%)
9.02
9.15
9.73
11.03
10.94
10.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CRT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CRT in samples. No significant cross-reactivity or interference between Rat CRT and analogues was observed.
