The Rat Cardiotrophin-1 (CT1) CLIA Kit is specifically designed for the accurate measurement of cardiotrophin-1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.Cardiotrophin-1 is a key cytokine that plays a critical role in heart development and function, as well as in the regulation of cardiac hypertrophy and remodeling. It is also involved in various physiological and pathological processes related to cardiovascular diseases.
Understanding the role of cardiotrophin-1 can provide valuable insights into these conditions and may lead to the development of potential therapeutic interventions.Overall, the Rat CT1 CLIA Kit from Assay Genie provides researchers with a powerful tool for studying the role of cardiotrophin-1 in cardiovascular diseases and other related conditions, ultimately contributing to the advancement of scientific knowledge and potential clinical applications.
Product Name:
Rat CT1 (Cardiotrophin 1) CLIA Kit
SKU:
RTES00092
Target:
Rat CT1 (Cardiotrophin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CT1. You can calculate the concentration of Rat CT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
87-102
85-96
Average (%)
96
94
91
1:4
Range (%)
91-106
103-116
104-118
Average (%)
97
108
110
1:8
Range (%)
102-115
101-117
97-111
Average (%)
108
109
102
1:16
Range (%)
84-98
100-116
103-116
Average (%)
90
109
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
83-95
90
Cell culture media (n=5)
91-106
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.17
69.36
204.94
26.3
73.94
198.86
Standard deviation
3.09
7.49
19.43
2.55
6.03
13.22
C V (%)
11.37
10.8
9.48
9.7
8.16
6.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CT1 in samples. No significant cross-reactivity or interference between Rat CT1 and analogues was observed.
