Rat CTACK (Cutaneous T-Cell Attracting Chemokine) CLIA Kit
The Rat Ctack (Cutaneous T-cell Attracting Chemokine) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Ctack levels in rat serum, plasma, and cell culture supernatants. This kit enables researchers to reliably measure Ctack levels, facilitating studies on inflammatory responses and immune cell migration.Ctack is a chemokine that plays a critical role in recruiting T-cells to the skin, regulating immune responses, and promoting inflammation.
Dysregulation of Ctack levels has been implicated in various inflammatory skin conditions, making it a valuable biomarker for studying these disorders and developing potential therapeutic interventions.With its high sensitivity and specificity, the Rat Ctack CLIA Kit offers researchers a precise and effective tool for investigating the role of Ctack in immune-mediated skin diseases and understanding the mechanisms underlying T-cell recruitment and inflammation in the skin.
Product Name:
Rat CTACK (Cutaneous T-Cell Attracting Chemokine) CLIA Kit
SKU:
RTES00158
Target:
Rat CTACK (Cutaneous T-Cell Attracting Chemokine)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CTACK. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTACK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTACK, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CTACK. You can calculate the concentration of Rat CTACK in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
98-111
101-117
Average (%)
98
104
107
1:4
Range (%)
92-104
103-120
86-99
Average (%)
99
110
92
1:8
Range (%)
102-118
90-103
86-100
Average (%)
110
97
93
1:16
Range (%)
86-100
95-107
84-100
Average (%)
92
101
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
101
EDTA plasma (n=5)
98-113
104
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.41
148.41
416.08
47.18
159.27
390.67
Standard deviation
5.69
15.94
42.65
4.29
18.32
39.15
C V (%)
12.26
10.74
10.25
9.09
11.5
10.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CTACK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTACK in samples. No significant cross-reactivity or interference between Rat CTACK and analogues was observed.
