Rat CTGF (Connective Tissue Growth Factor) CLIA Kit
The Rat CTGF (Connective Tissue Growth Factor) CLIA Kit is a highly sensitive and specific assay developed for the accurate measurement of CTGF levels in rat samples. This kit is designed for use in serum, plasma, and cell culture supernatants, providing reliable and reproducible results for a variety of research applications.CTGF is a key regulator of connective tissue growth and repair, playing a critical role in processes such as fibrosis, wound healing, and tissue remodeling.
Dysregulation of CTGF expression has been implicated in various diseases, including fibrotic disorders, osteoarthritis, and cancer. The Rat CTGF CLIA Kit is an essential tool for studying the role of CTGF in these conditions and for evaluating potential therapeutic interventions.
Product Name:
Rat CTGF (Connective Tissue Growth Factor) CLIA Kit
SKU:
RTES00148
Target:
Rat CTGF (Connective Tissue Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CTGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CTGF. You can calculate the concentration of Rat CTGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
96-111
89-104
Average (%)
90
103
96
1:4
Range (%)
100-118
95-112
91-108
Average (%)
109
103
98
1:8
Range (%)
93-108
99-117
90-102
Average (%)
100
107
95
1:16
Range (%)
94-105
103-119
101-114
Average (%)
99
110
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.25
80.58
352.82
40.41
73.85
332.88
Standard deviation
4.34
8.73
35.25
3.79
6.17
34.92
C V (%)
10.52
10.83
9.99
9.38
8.35
10.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CTGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTGF in samples. No significant cross-reactivity or interference between Rat CTGF and analogues was observed.
