The Rat CTSK (Cathepsin K) CLIA Kit is a highly reliable and accurate assay designed for the quantitative detection of Cathepsin K levels in rat serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, providing consistent and trustworthy results for various research applications.Cathepsin K is an important enzyme involved in bone resorption and remodeling, making it a key player in conditions such as osteoporosis and osteoarthritis.
By accurately measuring Cathepsin K levels, researchers can gain valuable insights into these diseases and potentially develop new therapeutic interventions.Overall, the Rat CTSK CLIA Kit is an essential tool for studying Cathepsin K biology and its role in various disease states, offering researchers a powerful tool for advancing their research efforts.
Product Name:
Rat CTSK (Cathepsin K) CLIA Kit
SKU:
RTES00102
Target:
Rat CTSK (Cathepsin K)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CTSK. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTSK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTSK, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CTSK. You can calculate the concentration of Rat CTSK in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
99-114
99-113
Average (%)
98
104
107
1:4
Range (%)
94-106
102-118
100-117
Average (%)
101
108
107
1:8
Range (%)
91-104
100-116
87-100
Average (%)
98
108
95
1:16
Range (%)
86-101
97-107
86-100
Average (%)
92
102
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.96
113.97
376.35
45.64
122.63
413.73
Standard deviation
5.93
7.98
23.6
5.22
10.09
30.2
C V (%)
12.36
7.0
6.27
11.44
8.23
7.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CTSK concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTSK in samples. No significant cross-reactivity or interference between Rat CTSK and analogues was observed.
