Rat CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit
The Rat CTX-II (Cross-linked C-telopeptide of Type II Collagen) ELISA Kit is specifically designed for the accurate measurement of CTX-II levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.CTX-II is a key biomarker associated with cartilage degradation and osteoarthritis, making it a valuable tool for studying joint health and potential treatments.
Monitoring CTX-II levels can provide important insights into disease progression and therapeutic efficacy, making this ELISA kit essential for researchers investigating musculoskeletal disorders.
Product Name:
Rat CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit
SKU:
RTES01165
Target:
Rat CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTXⅡ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTXⅡ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTXⅡ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CTXⅡ. You can calculate the concentration of Rat CTXⅡ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
95-106
95-111
Average (%)
94
100
102
1:4
Range (%)
87-101
86-98
83-95
Average (%)
92
91
89
1:8
Range (%)
87-101
86-100
83-93
Average (%)
92
91
88
1:16
Range (%)
91-102
81-95
89-104
Average (%)
96
88
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
98
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.19
4.33
0.43
1.31
4.48
Standard deviation
0.03
0.05
0.19
0.02
0.07
0.2
C V (%)
6.38
4.2
4.39
4.65
5.34
4.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CTXⅡ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTXⅡ in samples. No significant cross-reactivity or interference between Rat CTXⅡ and analogues was observed.
