Rat CTXI (Cross Linked C-telopeptide of Type I Collagen) CLIA Kit
The Rat CTXI (Cross-linked C-telopeptide of Type I Collagen) CLIA Kit is specifically designed for the quantitative measurement of CTXI levels in rat serum, plasma, and urine samples. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in various research applications.CTXI is a biomarker for bone resorption and is essential for studying bone-related diseases such as osteoporosis and bone metastasis.
Monitoring CTXI levels can provide valuable insights into bone turnover and the effectiveness of treatments targeting bone health.Overall, the Rat CTXI CLIA Kit is a valuable tool for researchers looking to investigate bone metabolism and assess the impact of various interventions on bone resorption levels in rat models.
Product Name:
Rat CTXI (Cross Linked C-telopeptide of Type I Collagen) CLIA Kit
SKU:
RTES00633
Target:
Rat CTXI (Cross Linked C-telopeptide of Type I Collagen)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CTXI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTXI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CTXI. You can calculate the concentration of Rat CTXI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
103-119
84-96
Average (%)
90
109
90
1:4
Range (%)
84-97
88-98
86-100
Average (%)
91
93
91
1:8
Range (%)
95-111
103-120
95-108
Average (%)
101
110
100
1:16
Range (%)
86-98
98-114
85-97
Average (%)
93
105
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.06
300.11
823.04
101.78
300.41
853.99
Standard deviation
12.11
26.44
52.02
11.55
29.71
56.96
C V (%)
11.42
8.81
6.32
11.35
9.89
6.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CTXI concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTXI in samples. No significant cross-reactivity or interference between Rat CTXI and analogues was observed.
