The Rat Cx26 (Connexin 26) CLIA Kit is a cutting-edge assay designed for the accurate measurement of Connexin 26 levels in rat samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for research purposes. Connexin 26 is a key protein involved in cell-to-cell communication via gap junctions, playing a crucial role in various physiological processes. Dysregulation of Connexin 26 has been linked to conditions like deafness, skin disorders, and certain genetic diseases, making it a valuable biomarker for studying these conditions and potential therapeutic targets.
With its advanced technology and reliable performance, the Rat Cx26 (Connexin 26) CLIA Kit is a valuable tool for researchers working in the fields of cell biology, physiology, and molecular biology. Get accurate and insightful results with this innovative assay kit from Assay Genie.
Product Name:
Rat CX26 (Connexin 26) CLIA Kit
SKU:
RTES00149
Target:
Rat CX26 (Connexin 26)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CX26. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CX26 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CX26, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CX26. You can calculate the concentration of Rat CX26 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
92-109
94-108
Average (%)
96
99
99
1:4
Range (%)
95-109
94-106
85-99
Average (%)
102
99
91
1:8
Range (%)
101-115
85-97
85-99
Average (%)
107
91
91
1:16
Range (%)
95-110
101-118
89-105
Average (%)
101
108
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
97-110
103
Cell culture media (n=5)
98-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.98
47.39
174.75
25.39
44.48
182.23
Standard deviation
2.05
3.42
16.44
2.97
4.68
19.64
C V (%)
8.55
7.22
9.41
11.7
10.52
10.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CX26 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CX26 in samples. No significant cross-reactivity or interference between Rat CX26 and analogues was observed.
