Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) CLIA Kit
The Rat CX3CL1 (Chemokine C-X3-C motif ligand 1) CLIA Kit is specifically designed for the precise measurement of CX3CL1 levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.CX3CL1, also known as fractalkine, is a unique chemokine that plays a key role in immune cell recruitment and adhesion in various inflammatory processes.
Its dysregulation has been implicated in a range of diseases, including neuroinflammatory disorders, cardiovascular conditions, and cancer. The Rat CX3CL1 CLIA Kit enables researchers to study the involvement of CX3CL1 in these pathological conditions and explore potential therapeutic interventions.
Product Name:
Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) CLIA Kit
SKU:
RTES00213
Target:
Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CX3CL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CX3CL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CX3CL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CX3CL1. You can calculate the concentration of Rat CX3CL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
92-109
98-113
Average (%)
92
99
103
1:4
Range (%)
87-101
92-103
97-111
Average (%)
94
98
103
1:8
Range (%)
101-117
98-113
92-104
Average (%)
109
105
98
1:16
Range (%)
95-109
102-118
88-100
Average (%)
102
108
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
97
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.1
261.11
698.69
98.92
250.55
757.52
Standard deviation
10.13
30.24
69.87
9.6
21.22
59.84
C V (%)
9.55
11.58
10.0
9.7
8.47
7.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CX3CL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CX3CL1 in samples. No significant cross-reactivity or interference between Rat CX3CL1 and analogues was observed.
