The Rat CX40 (Connexin 40) CLIA Kit is a powerful tool for the quantitative measurement of Connexin 40 levels in rat samples. This kit is optimized for use with rat serum, plasma, and tissue homogenates, offering high sensitivity and accuracy for reliable results.Connexin 40 is a key protein involved in gap junction communication between cells, playing a critical role in cardiovascular function and electrical conduction in the heart. Dysregulation of Connexin 40 has been associated with various cardiovascular disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic targets.
With its user-friendly protocol and robust performance, the Rat CX40 CLIA Kit is an essential tool for researchers investigating the role of Connexin 40 in cardiovascular health and disease. Get accurate and consistent results with this reliable assay kit from Assay Genie.
Product Name:
Rat CX40 (Connexin 40) CLIA Kit
SKU:
RTES00150
Target:
Rat CX40 (Connexin 40)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CX40. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CX40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CX40, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CX40. You can calculate the concentration of Rat CX40 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
95-108
90-106
Average (%)
101
101
97
1:4
Range (%)
97-112
92-108
85-101
Average (%)
103
98
92
1:8
Range (%)
90-103
92-108
97-111
Average (%)
97
99
105
1:16
Range (%)
87-100
86-99
102-117
Average (%)
94
91
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
94-110
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.06
87.13
378.78
44.36
90.07
365.67
Standard deviation
5.0
6.4
29.62
4.12
9.71
27.21
C V (%)
11.61
7.35
7.82
9.29
10.78
7.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CX40 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CX40 in samples. No significant cross-reactivity or interference between Rat CX40 and analogues was observed.
