The Rat CX43 (Connexin 43) CLIA Kit is a highly reliable and sensitive assay designed for the quantitative detection of connexin 43 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional accuracy and reproducibility, making it an essential tool for researchers studying cellular communication and intercellular signaling pathways.Connexin 43 is a critical protein that forms gap junctions between cells, allowing for the direct exchange of ions, metabolites, and signaling molecules. Dysregulation of connexin 43 has been implicated in various pathological conditions, including cardiovascular diseases, neurological disorders, and cancer, underscoring its importance as a biomarker for understanding disease mechanisms and developing targeted therapies.
With its high sensitivity and specificity, the Rat CX43 CLIA Kit provides researchers with a valuable tool for investigating connexin 43 expression levels and function in experimental models, ultimately contributing to advancements in our understanding of cell-cell communication and its role in health and disease.
Product Name:
Rat CX43 (Connexin 43) CLIA Kit
SKU:
RTES00151
Target:
Rat CX43 (Connexin 43)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CX43. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CX43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CX43, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CX43. You can calculate the concentration of Rat CX43 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
95-107
93-108
Average (%)
108
102
100
1:4
Range (%)
93-106
91-105
103-119
Average (%)
99
97
110
1:8
Range (%)
88-100
96-111
102-120
Average (%)
95
102
110
1:16
Range (%)
93-108
87-101
93-109
Average (%)
100
93
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
97-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.52
306.71
954.03
108.55
279.35
962.75
Standard deviation
10.48
28.95
97.12
10.05
30.9
65.85
C V (%)
9.84
9.44
10.18
9.26
11.06
6.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CX43 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CX43 in samples. No significant cross-reactivity or interference between Rat CX43 and analogues was observed.
