Rat CXCL16 (Chemokine C-X-C-Motif Ligand 16) CLIA Kit
The Rat CXCL16 (Chemokine C-X-C Motif Ligand 16) CLIA Kit is a high-quality assay designed for the accurate detection of CXCL16 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CXCL16 is an important chemokine involved in immune responses and inflammatory processes. Its role in promoting recruitment and activation of immune cells makes it a key player in various diseases, such as autoimmune disorders, inflammatory conditions, and cancer.
Studying CXCL16 levels can provide valuable insights into these diseases and aid in the development of potential therapeutic strategies.Overall, the Rat CXCL16 CLIA Kit is a powerful tool for researchers seeking to investigate the role of CXCL16 in health and disease, offering reliable and accurate measurement of this important chemokine in rat samples.
Product Name:
Rat CXCL16 (Chemokine C-X-C-Motif Ligand 16) CLIA Kit
SKU:
RTES00109
Target:
Rat CXCL16 (Chemokine C-X-C-Motif Ligand 16)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CXCL16. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CXCL16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CXCL16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CXCL16. You can calculate the concentration of Rat CXCL16 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
103-119
95-110
Average (%)
101
109
102
1:4
Range (%)
101-118
91-103
93-111
Average (%)
108
96
101
1:8
Range (%)
99-117
84-97
102-115
Average (%)
107
91
108
1:16
Range (%)
102-120
100-112
88-104
Average (%)
110
105
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
105
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.64
238.33
955.73
86.79
222.14
861.87
Standard deviation
9.94
16.85
77.89
8.62
23.68
90.93
C V (%)
10.5
7.07
8.15
9.93
10.66
10.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CXCL16 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CXCL16 in samples. No significant cross-reactivity or interference between Rat CXCL16 and analogues was observed.
