Rat CXCL5 (Chemokine C-X-C-Motif Ligand 5) ELISA Kit
The Rat CXCL5 (Chemokine C-X-C-Motif Ligand 5) ELISA Kit is a high-performance tool designed for the precise and quantitative measurement of CXCL5 levels in a variety of rat biological samples. CXCL5, a member of the C-X-C chemokine family, is a potent chemoattractant involved in mediating inflammatory responses and regulating immune cell migration. Known for its critical role in various immune processes and inflammation, CXCL5 serves as a key player in orchestrating cellular signaling pathways and modulating immune cell functions. This ELISA kit enables researchers to accurately quantify CXCL5 concentrations, providing valuable insights into its involvement in chemotaxis, immune cell recruitment, and inflammatory cascades.
By elucidating the role of CXCL5 in immune modulation and inflammatory pathways, researchers can gain a deeper understanding of its significance in disease pathogenesis, immunity, and potential therapeutic interventions. Crafted with precision and reliability, the Rat CXCL5 ELISA Kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results. Engineered under stringent quality control measures, this kit delivers robust performance and ease of use, making it an ideal choice for research endeavors seeking to elucidate the intricate mechanisms regulated by CXCL5 in physiological and pathological conditions.
Product Name:
Rat CXCL5 (Chemokine C-X-C-Motif Ligand 5) ELISA Kit
SKU:
AEES00553
Target:
Rat CXCL5 (Chemokine C-X-C-Motif Ligand 5)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CXCL5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CXCL5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CXCL5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CXCL5. You can calculate the concentration of Rat CXCL5 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
86-97
86-100
Average (%)
91
92
93
1:4
Range (%)
82-97
88-102
86-101
Average (%)
89
94
93
1:8
Range (%)
86-100
87-102
82-95
Average (%)
92
93
89
1:16
Range (%)
82-94
81-93
87-100
Average (%)
87
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
83-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
0.92
3.57
0.43
0.9
3.34
Standard deviation
0.03
0.04
0.13
0.03
0.05
0.15
C V (%)
6.72
4.16
3.64
6.18
5.15
4.38
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CXCL5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CXCL5 in samples. No significant cross-reactivity or interference between Rat CXCL5 and analogues was observed.
