Description
Rat Cyclin-dependent kinase 5 activator 1 (Cdk5r1) ELISA Kit
The Rat Cyclin-Dependent Kinase 5 Activator 1 (CDK5R1) ELISA Kit is a reliable tool for the accurate measurement of CDK5R1 levels in rat samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it perfect for various research applications.CDK5R1 is a key protein that regulates the activity of Cyclin-Dependent Kinase 5 (CDK5), playing a critical role in neuronal development, synaptic plasticity, and neurodegenerative diseases.
By studying CDK5R1 levels, researchers can gain insights into various neurological disorders and potential therapeutic strategies.Overall, the Rat CDK5R1 ELISA Kit is a valuable tool for studying the functions and implications of CDK5R1 in rat models, providing researchers with valuable data for their studies in neuroscience and related fields.
| Product Name: | Rat Cyclin-dependent kinase 5 activator 1 (Cdk5r1) ELISA Kit |
| SKU: | RTEB1078 |
| Size: | 96T |
| Target: | Rat Cyclin-dependent kinase 5 activator 1 (Cdk5r1) |
| Synonyms: | Cyclin-dependent kinase 5 regulatory subunit 1, TPKII regulatory subunit, CDK5 activator 1, Cdk5r |
| Assay Type: | Sandwich |
| Detection Method: | ELISA |
| Reactivity: | Rat |
| Detection Range: | 0.156-10ng/ml |
| Sensitivity: | 0.093ng/mL |
| Intra CV: | 4.4% | ||||||||||||||||||||
| Inter CV: | 8.4% | ||||||||||||||||||||
| Linearity: |
| ||||||||||||||||||||
| Recovery: |
| ||||||||||||||||||||
| Function: | p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution. |
| Uniprot: | P61810 |
| Sample Type: | Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids |
| Specificity: | Natural and recombinant rat Cyclin-dependent kinase 5 activator 1 |
| Sub Unit: | Heterodimer composed of CDK5 and CDK5R (p25) and macromolecular complex composed of at least CDK5, CDK5R (p35) and CDK5RAP1 or CDK5RAP2 or CDK5RAP3. Only the heterodimer shows kinase activity (By similarity). Interacts with RASGRF2. Interacts with EPHA4 and NGEF; may mediate the activation of NGEF by EPHA4. The complex p35/CDK5 interacts with CLOCK. |
| Research Area: | Cancer |
| Subcellular Location: | Cyclin-dependent kinase 5 activator 1, p25 Nucleus Cytoplasm Perinuclear region The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. |
| Storage: | Please see kit components below for exact storage details |
| Note: | For research use only |
| UniProt Protein Function: | CDK5R1: activator of cyclin-dependent kinase 5 (CDK5). Required for proper development of the central nervous system. The p35 form of this protein is cleaved by calpain, generating a p25 form, resulting in the prolonged activation and relocalization of the CDK5 from the cell periphery to nuclear and perinuclear regions. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease, leading to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. |
| UniProt Protein Details: | Protein type:Protein kinase, regulatory subunit Chromosomal Location of Human Ortholog: 10q26 Cellular Component: axon; cell soma; contractile fiber; cyclin-dependent protein kinase 5 activator complex; cytoplasm; dendrite; dendritic spine; growth cone; intracellular membrane-bound organelle; membrane; neuromuscular junction; nucleoplasm; nucleus; perinuclear region of cytoplasm; postsynaptic density Molecular Function:cadherin binding; calcium ion binding; cyclin-dependent protein kinase 5 activator activity; ephrin receptor binding; kinase activity; protein binding; protein kinase binding; protein serine/threonine kinase activator activity Biological Process: acetylcholine receptor signaling, muscarinic pathway; axon extension; axon guidance; axonal fasciculation; brain development; cerebellum development; cerebral cortex radially oriented cell migration; ephrin receptor signaling pathway; hippocampus development; ionotropic glutamate receptor signaling pathway; layer formation in the cerebral cortex; negative regulation of axon extension; negative regulation of transcription, DNA-dependent; neurite development; neuron adhesion; neuron differentiation; neuron migration; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of neuron apoptosis; positive regulation of protein kinase activity; regulation of actin cytoskeleton organization and biogenesis; serine phosphorylation of STAT protein; superior olivary nucleus maturation |
| NCBI Summary: | kinase that phosphorylates neurofilament protein (NF-H)and intermediate filament protein nestin [RGD, Feb 2006] |
| UniProt Code: | P61810 |
| NCBI GenInfo Identifier: | 16758762 |
| NCBI Gene ID: | 116671 |
| NCBI Accession: | NP_446343.1 |
| UniProt Secondary Accession: | P61810,Q62938, |
| UniProt Related Accession: | P61810 |
| Molecular Weight: | 34,031 Da |
| NCBI Full Name: | cyclin-dependent kinase 5 activator 1 |
| NCBI Synonym Full Names: | cyclin-dependent kinase 5 regulatory subunit 1 |
| NCBI Official Symbol: | Cdk5r1 |
| NCBI Official Synonym Symbols: | p35; Cdk5r |
| NCBI Protein Information: | cyclin-dependent kinase 5 activator 1 |
| UniProt Protein Name: | Cyclin-dependent kinase 5 activator 1 |
| UniProt Synonym Protein Names: | Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit |
| Protein Family: | Cyclin-dependent kinase 5 activator |
| UniProt Gene Name: | Cdk5r1 |
| Component | Quantity (96 Assays) | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | -20°C |
| Lyophilized Standard | 2 | -20°C |
| Sample Diluent | 20ml | -20°C |
| Assay Diluent A | 10mL | -20°C |
| Assay Diluent B | 10mL | -20°C |
| Detection Reagent A | 120µL | -20°C |
| Detection Reagent B | 120µL | -20°C |
| Wash Buffer | 30mL | 4°C |
| Substrate | 10mL | 4°C |
| Stop Solution | 10mL | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.
| Step | |
| 1. | Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C. |
| 2. | Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform. |
| 3. | Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper. |
| 4. | Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C. |
| 5. | Repeat the wash process for five times as conducted in step 3. |
| 6. | Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction. |
| 7. | Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing. |
| 8. | Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters. |
| 9. | After experiment, store all reagents according to the specified storage temperature respectively until their expiry. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
