Rat CYP2E1 (Cytochrome P450, family 2, subfamily E, polypeptide 1) CLIA Kit
The Rat CYP2E1 (Cytochrome P450 Family 2 Subfamily E Polypeptide 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of rat CYP2E1 levels in biological samples. This kit is suitable for use with rat serum, plasma, and tissue homogenates, providing researchers with reliable and reproducible results for their experiments.CYP2E1 is a key enzyme in the cytochrome P450 family responsible for metabolism and detoxification of various xenobiotics, including drugs and environmental toxins.
Dysregulation of CYP2E1 has been implicated in numerous health conditions, such as liver disease, diabetes, and cancer, making it a valuable target for drug development and disease research.With its superior performance and easy-to-use format, the Rat CYP2E1 CLIA Kit is an essential tool for studying the role of CYP2E1 in health and disease, and for identifying potential therapeutic interventions targeting this important enzyme. Trust AssayGenie for all your research needs.
Product Name:
Rat CYP2E1 (Cytochrome P450, family 2, subfamily E, polypeptide 1) CLIA Kit
SKU:
RTES00170
Target:
Rat CYP2E1 (Cytochrome P450, family 2, subfamily E, polypeptide 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CYP2E1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CYP2E1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CYP2E1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CYP2E1. You can calculate the concentration of Rat CYP2E1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
92-103
99-111
Average (%)
105
98
105
1:4
Range (%)
86-100
91-102
93-105
Average (%)
92
96
99
1:8
Range (%)
92-109
101-113
86-99
Average (%)
100
107
93
1:16
Range (%)
91-105
85-99
96-108
Average (%)
96
91
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
93
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
102-114
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.15
4.45
14.26
2.02
4.89
14.88
Standard deviation
0.26
0.36
1.17
0.18
0.37
1.28
C V (%)
12.09
8.09
8.2
8.91
7.57
8.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CYP2E1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CYP2E1 in samples. No significant cross-reactivity or interference between Rat CYP2E1 and analogues was observed.
