The Rat CypB (Cyclophilin B) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of Cyclophilin B levels in rat samples including serum, plasma, and cell culture supernatants. With reliable and reproducible results, this kit is ideal for a wide range of research applications.Cyclophilin B is a key protein involved in various cellular processes, including protein folding and trafficking. Dysregulation of Cyclophilin B has been linked to several pathological conditions such as cancer, autoimmune diseases, and viral infections, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
Overall, the Rat CypB CLIA Kit offers researchers a powerful tool to investigate the role of Cyclophilin B in disease pathogenesis and explore its potential as a therapeutic target.
Product Name:
Rat CYPB (Cyclophilin B) CLIA Kit
SKU:
RTES00166
Target:
Rat CYPB (Cyclophilin B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CYPB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CYPB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CYPB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CYPB. You can calculate the concentration of Rat CYPB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
99-116
92-106
Average (%)
101
106
100
1:4
Range (%)
96-114
93-106
95-109
Average (%)
104
100
101
1:8
Range (%)
88-103
99-113
93-107
Average (%)
94
107
101
1:16
Range (%)
87-98
104-117
85-98
Average (%)
92
110
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
90-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.86
5.27
19.17
1.89
4.8
19.62
Standard deviation
0.22
0.45
2.19
0.24
0.44
1.32
C V (%)
11.83
8.54
11.42
12.7
9.17
6.73
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CYPB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CYPB in samples. No significant cross-reactivity or interference between Rat CYPB and analogues was observed.
