Rat CYSLTR2 (Cysteinyl Leukotriene Receptor 2) CLIA Kit
The Rat CysLTR2 (Cysteinyl Leukotriene Receptor 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of CysLTR2 levels in rat samples. This kit is ideal for research applications where the role of CysLTR2 in inflammation, asthma, and other related conditions is of interest.Cysteinyl Leukotriene Receptor 2 is a key receptor involved in the inflammatory response, particularly in respiratory diseases such as asthma.
By measuring the levels of CysLTR2 in rat samples, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets. The Rat CysLTR2 CLIA Kit offers reliable and reproducible results, making it a valuable tool for studying the role of CysLTR2 in disease pathology and drug development.
Product Name:
Rat CYSLTR2 (Cysteinyl Leukotriene Receptor 2) CLIA Kit
SKU:
RTES00168
Target:
Rat CYSLTR2 (Cysteinyl Leukotriene Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CYSLTR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CYSLTR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CYSLTR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CYSLTR2. You can calculate the concentration of Rat CYSLTR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
92-108
90-105
Average (%)
106
99
97
1:4
Range (%)
91-101
100-118
89-102
Average (%)
96
108
94
1:8
Range (%)
89-103
98-111
98-115
Average (%)
96
105
106
1:16
Range (%)
85-101
100-120
99-111
Average (%)
92
109
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
97-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
209.44
356.45
1389.44
228.42
381.21
1511.49
Standard deviation
16.99
34.01
126.3
26.95
40.03
155.38
C V (%)
8.11
9.54
9.09
11.8
10.5
10.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CYSLTR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CYSLTR2 in samples. No significant cross-reactivity or interference between Rat CYSLTR2 and analogues was observed.
