Rat Deiodinase, Iodothyronine, Type II (DIO2) ELISA Kit
The Rat Deiodinase Iodothyronine Type II (DIO2) ELISA Kit is a powerful tool for accurately measuring levels of DIO2 in rat samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers precise and reproducible results, making it perfect for a variety of research applications.DIO2 is an enzyme crucial for thyroid hormone metabolism, playing a key role in regulating thyroid hormone levels in the body. Dysregulation of DIO2 has been linked to thyroid disorders, obesity, and cardiovascular disease, making it a valuable biomarker for studying these conditions and developing potential treatments.
By using the Rat Deiodinase Iodothyronine Type II (DIO2) ELISA Kit, researchers can gain valuable insights into thyroid hormone regulation and its impact on various physiological processes, ultimately advancing our understanding of thyroid-related diseases and potential therapeutic interventions.
Product Name:
Rat Deiodinase, Iodothyronine, Type II (DIO2) ELISA Kit
Product Code:
RTDL00300
Size:
96 Assays
Target:
DIO2
Synonyms:
5DII, D2, SelY, TXDI2, Thyroxine Deiodinase,Type II, Type-II 5'-deiodinase
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
